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目的探讨骨髓间充质干细胞(Bone marrow mesenchymal stem cells,BMSCs)移植和直流电刺激对大鼠脊髓损伤(Spinal cord injury,SCI)的修复作用。方法采用脊椎骨破坏法制备大鼠SCI模型,将模型大鼠随机分为模型组、BMSCs移植组、电刺激组和电刺激+BMSCs移植组,通过BBB评分、体感诱发电位(Somatosensory evoked potential,SEP)测定和免疫组化法观察不同方法治疗SCI的效果。结果 BMSCs移植后7和14 d,模型组和电刺激组BBB评分与BMSCs移植组和电刺激+BM-SCs移植组相比,差异有统计学意义(P<0.05);电刺激+BMSCs移植组脊髓功能恢复最好。SEP检测,BMSCs移植后14 d,BMSCs移植组和电刺激+BMSCs移植组与其他两组比较,潜伏期幅有明显差异(P<0.05)。采用NF-200标记脊髓神经元,模型组在损伤14 d后,仅见少量的NF阳性细胞;而电刺激组和BMSCs移植组可见大量NF阳性细胞和轴突分布于损伤区周围;电刺激+BMSCs移植组NF阳性细胞明显多于电刺激组和BMSCs移植组(P<0.05)。BMSCs移植组在移植后7 d,局部组织脑衍生的亲神经因子(Brain-derived neurotropic factor,BDNF)表达量明显高于模型组(P<0.05),14 d最强,21 d减弱,电刺激+BMSCs移植组BDNF表达最强(P<0.05)。结论 BMSCs移植联合电刺激能够最大程度地促进SCI修复。
Objective To investigate the effect of Bone marrow mesenchymal stem cells (BMSCs) transplantation and DC stimulation on the repair of Spinal cord injury (SCI) in rats. Methods Sprague-Dawley rats were used to establish SCI model. The model rats were randomly divided into model group, BMSCs transplantation group, electrical stimulation group and BMSCs transplantation group. BBB score, somatosensory evoked potential (SEP) Determination and immunohistochemistry observed different methods of treatment of SCI effect. Results At 7 and 14 days after transplantation, the BBB score of model group and electrical stimulation group was significantly higher than that of BMSCs transplantation group and electrical stimulation + BM-SCs transplantation group (P <0.05); BMSCs transplantation group Spinal cord function recovery is best. Compared with the other two groups, the latency of the BMSCs transplantation group and the BMSCs transplantation group were significantly different (P <0.05) by SEP. Spinal cord neurons were labeled with NF-200. Only a few NF-positive cells were seen in the model group at 14 days after injury. However, a large number of NF-positive cells and axons were found in the injured area around the electrical stimulation group and BMSCs transplantation group. The number of NF positive cells in the transplantation group was significantly more than that in the electrical stimulation group and BMSCs transplantation group (P <0.05). At 7 days after transplantation, the expression of brain-derived neurotropic factor (BDNF) in BMSCs transplantation group was significantly higher than that in model group (P <0.05) + BMSCs transplantation BDNF expression was the strongest (P <0.05). Conclusion BMSCs transplantation combined with electrical stimulation can promote SCI repair to the maximum extent.