采用硫酸-苯酚法,以无水葡萄糖为对照品,于485nm处测定吸光度,测定秋海棠药材中多糖的含量。在3.01—10.54μg.mL-1范围内吸光度与葡萄糖溶液浓度的线性关系良好(r=0.9997),平均回收率为98.06%,RSD为0.50%。该方法简便快速、准确可行,为秋海棠药材的质量控制提供了实验依据。 Using sulfuric acid - phenol method to anhydrous glucose as a reference substance, measured at 485nm absorbance determination Begonia medicinal polysaccharide content. The linear relationship between absorbance and concentration of glucose solution was good (r = 0.9997) in the range of 3.01-10.54μg.mL-1 with the average recovery of 98.06% and RSD 0.50%. The method is simple and rapid, accurate and feasible, which provides the experimental basis for the quality control of Begonia.