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本文以液体闪烁谱仪计数法,测定离体缺血再灌注鼠心肌线粒体内~(45)Ca~(2+)的放射强度(cpm),观察ATP-MgCl_2、超氧化物歧化酶(SOD)和异搏定对缺血再灌注鼠心肌线粒体内钙离子的影响。结果表明,ATP-MgCl_2,SOD和异搏定组的~(45)Ca~(2+)cpm显著低于对照组的~(45)Ca~(2+)cpm(P<0.01),ATP-MgCl_2,组和SOD组的~(45)Ca~2+cpm又低于异搏定组的~(45)Ca~(2+)cpm,而ATP-MgCl_2组和SOD组之间无显著性差异(P>0.05),结果提示,ATP-MgCl_2、SOD和异搏定三种药物均能阻止离体大鼠心肌线柱体内Ca~(2+)积聚,从而保护心肌细胞缺血再灌注性损伤。
In this paper, the radioactive intensity (cpm) of ~(45)Ca~(2+) in myocardial mitochondria was determined by liquid scintillation spectrometry, and the ATP-MgCl 2 and superoxide dismutase (SOD) were observed. Effect of verapamil on the mitochondrial calcium ion in myocardial ischemia / reperfusion in. The results showed that the ~(45)Ca~(2+)cpm of ATP-MgCl2, SOD and verapamil were significantly lower than the ~(45)Ca~(2+)cpm (P<0.01), ATP- The ~(45)Ca~2+cpm of MgCl2, group and SOD group was lower than that of verapamil group ~(45)Ca~(2+)cpm, but there was no significant difference between ATP-MgCl2 group and SOD group. (P>0.05) The results suggest that ATP-MgCl 2 , SOD, and verapamil can all prevent the accumulation of Ca 2+ in the isolated rat myocardial column, thus protecting the myocardial cell from ischemia-reperfusion injury. .