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本文研究了硼对灰霉病菌孢子萌发的影响并对其抑制机理作了进一步的分析。用0.1%硼处理灰霉病菌孢子后,显著地抑制了孢子萌发和芽管伸长。与不处理的对照相比,0.1%硼使灰霉病菌孢子细胞内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)的活性逐渐下降,还原型谷胱甘肽(GSH)和抗坏血酸(ASA)含量逐渐降低,丙二醛(MDA)、过氧化氢(H2O2)含量和超氧阴离子(O.-2)产生速率上升。此外,DCHF-DA荧光染色结果也进一步证明硼处理造成了灰霉病菌孢子细胞内活性氧的积累。本试验结果初步表明,0.1%硼处理使细胞内活性氧清除系统破坏,膜脂过氧化作用加强,是抑制灰霉病菌孢子萌发和芽管伸长的重要原因。
In this paper, the effect of boron on the spore germination of Botrytis cinerea was studied and its inhibition mechanism was further analyzed. Treatment of Botrytis cinerea spores with 0.1% boron significantly inhibited spore germination and germ tube elongation. 0.1% boron decreased the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in the spores of Botrytis cinerea compared to the untreated control, The content of GSH and ASA decreased gradually, and the content of malondialdehyde (MDA), hydrogen peroxide (H2O2) and the production rate of superoxide anion (-0.2) increased. In addition, DCHF-DA fluorescence staining results further confirmed that boron treatment caused the accumulation of reactive oxygen species in the spores of Botrytis cinerea. The results of this experiment showed that 0.1% boron treatment can destroy the active oxygen scavenging system and enhance the membrane lipid peroxidation, which is an important reason to inhibit spore germination and germ tube elongation of Botrytis cinerea.