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目的探索大鼠急性肺损伤/急性呼吸窘迫综合征(ALI/ARDS)的自由基变化及早期应用自由基清除剂松果菊苷(ECH)的防护作用。方法本研究分设4个研究组:对照组、ECH组、油酸(OA)组、OA-ECH组,分别在5个时间点采取标本检测动脉血气、肺组织活性氧(ROS)信号、肺湿/干重比值和肺病理。结果在各组中ALI的程度与自由基的相对浓度具有一致性,自由基的改变(30min)明显早于肺组织病理学(2h)改变。在注射OA后2h,OA组肺自由基达130～140高斯(Gauss),氧分压降至45～55mmHg;而OA-ECH组自由基是70～80Gauss,氧分压在65～75mmHg。肺湿/干重比值和病理学评分在2组差异也有统计学意义(P<0.01)。ECH通过减少ROS的生成,可明显减轻ALI的病理改变和低氧血症。结论 ROS是ALI发病过程的启动因子,早期应用抗氧化剂ECH可在一定程度上改善ALI的预后。
Objective To explore the changes of free radical in rat acute lung injury / acute respiratory distress syndrome (ALI / ARDS) and the protective effect of early free radical scavenger echinacoside (ECH). Methods The study was divided into four groups: control group, ECH group, OA group and OA-ECH group. Blood samples were taken from the arterial blood gas, lung tissue reactive oxygen species (ROS) / Dry weight ratio and lung pathology. Results The levels of ALI in all groups were consistent with the relative concentrations of free radicals. The change of free radicals (30min) was significantly earlier than that of lung histopathology (2h). At 2h after injection of OA, the free radicals in OA group reached 130-140 Gauss and the partial pressure of oxygen dropped to 45-55mmHg. In OA-ECH group, the free radicals were 70-80Gauss and the partial pressure of oxygen was 65-75mmHg. Lung wet / dry weight ratio and pathological score in the two groups were also statistically significant (P <0.01). ECH can significantly reduce the pathological changes of ALI and hypoxemia by reducing the generation of ROS. Conclusion ROS is the initiating factor in the pathogenesis of ALI. Early use of antioxidant ECH can improve the prognosis of ALI to a certain extent.