用β-ＢＧＴ结合蛋白兔疫Ｂａｌｂ／Ｃ小鼠、以免疫酶点技术（ＥＬＩＳＰＯＴ）和ＥＬＩＳＡ方法观察免疫后１～８周小鼠脾脏中特异性抗体分泌细胞和相应时期血清抗体的产生情况。结果免疫后２周小鼠脾脏中即产生特异性ＩｇＧ型抗体分泌细胞，７周以后渐趋消失；血清中ＩｇＧ抗体滴度则在第５周以后才开始增高，并持续在恒定水平至８周以后；实验观察期间仅见小鼠毛发枯干、活动少现象，但未见明显肌无力症状出现。上述结果提示：β-ＢＧＴ结合蛋白具有较好的免疫原性，但在重症肌无力（ＭＧ）发病中的致病作用则有待进一步研究。 Balb / C mice were immunized with β-BGT binding protein and the secreted cells in the spleen of mice immunized with ELISPOT and ELISA were observed at 1 to 8 weeks after immunization. Results After 2 weeks of immunization, specific IgG antibody secreting cells were produced in the spleens of mice and gradually disappeared after 7 weeks. The serum IgG antibody titers began to increase after 5 weeks and continued at a constant level to 8 weeks Later; during the experimental observation, only withered mice hair, less activity, but no obvious symptoms of muscle weakness. These results suggest that: β-BGT binding protein has good immunogenicity, but the pathogenesis of myasthenia gravis (MG) remains to be further studied.