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以胶质芽孢杆菌(Bacillus mucilaginosus)和棘孢曲霉(Aspergillus aculeatus)分别作为细菌和真菌的代表,进行了黑云母的生物风化实验(为期50 d),研究了微生物作用下黑云母中元素溶出速率和溶出顺序,并对溶解机制进行了初步探讨。利用高效液相色谱仪和电感耦合等离子体发射光谱仪分别测定了培养液中的低分子量有机酸和主要元素(Si、Al、Fe、Mg和K)的浓度。研究结果表明:胶质芽孢杆菌和棘孢曲霉均可促进黑云母的风化;棘孢曲霉的风化速率大约是胶质芽孢杆菌的65倍;真菌与细菌作用下黑云母中元素溶出顺序基本相同,即:层间→八面体晶片→四面体晶片;微生物主要通过分泌低分子量有机酸(酸化环境和络合)来促进黑云母的风化。
The biotinylation experiments of biotite (50 days) were carried out using Bacillus mucilaginosus and Aspergillus aculeatus as the representatives of bacteria and fungi respectively. The dissolution rate of biotite in biotite And dissolution order, and the dissolution mechanism was discussed. The concentrations of low molecular weight organic acids and major elements (Si, Al, Fe, Mg and K) in the medium were measured by high performance liquid chromatography and inductively coupled plasma atomic emission spectrometry. The results showed that both Bacillus cereus and Aspergillus aculeatus could accelerate the weathering of biotite; the rate of weevils of Aspergillus aculeatus was about 65 times that of Bacillus cereus; the order of dissolution of biotite in fungi and bacteria was basically the same, That is, between layers → octahedral chips → tetrahedral chips; microorganisms promote the weathering of biotite mainly through the secretion of low molecular weight organic acids (acidified environment and complexation).