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用高胚性细胞系Treki作为筛选材料，进行了抗乙硫氨酸（ETH）变异体的初步筛选。愈伤组织经2、4、6KRγ射线照射后，转到含不同浓度的ETH固体和液体选择培养基中，继代筛选三个月后，抗性愈伤组织中的含硫氨基酸量比对照提高1．05～1．84倍，并在0．02－1．0mMETH范围内表现出有随ETH浓度升高而逐渐提高的趋势。从0．02、0．01mMETH的抗性愈伤组织获得了一批再生植株．植株体内的含硫氨基酸量是对照的1．83和4．17倍。结果表明细胞水平的突变体筛选，有可能是一条改良苜蓿品质的育种途径。
Preliminary screening of anti-ethionine (ETH) variants was performed using the high-embryogenic cell line Treki as a screening material. The callus was irradiated with 2, 4, 6KRγγ rays and then transferred to ETH containing different concentrations of solid and liquid selection medium. After subculture for three months, the amount of sulfur-containing amino acids in the resistant callus was higher than that of the control 1.05 ~ 1.84 times, and in the range of 0.02-1.0ETETH showed a trend of increasing gradually with increasing ETH concentration. A number of regenerated plants were obtained from resistant calli at 0.02, 0.01 mM ETH. The amount of sulfur-containing amino acids in plants was 1.83 and 4.17 times that of the control. The results showed that the screening of mutant cells at the cellular level may be a breeding method to improve the quality of alfalfa.