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以“津春二号”黄瓜为试材,研究了硝酸钙胁迫下腐胺对黄瓜幼苗生长、抗氧化系统及光合作用的影响。结果表明:与对照相比,硝酸钙胁迫导致黄瓜幼苗叶片超氧阴离子产生速率、过氧化氢和丙二醛含量、膜透性显著升高,其净光合速率、气孔导度、蒸腾速率、胞间CO2浓度分别下降33.39%、55.18%、30.23%、34.56%,幼苗干物质积累减少44.17%,生长显著受抑;而腐胺可提高叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)等抗氧化酶活性,降低其超氧阴离子产生速率、过氧化氢和丙二醛(MDA)含量以及细胞膜透性,缓解硝酸钙胁迫下Pn值下降幅度,幼苗干物质积累增加21.59%,加快生长;可见,腐胺可通过调节硝酸钙胁迫下黄瓜幼苗抗氧化性以减少其膜脂过氧化程度,进而维持其较高的光合性能,有效促进了硝酸钙胁迫下黄瓜幼苗生长。
The effects of putrescine on the growth, antioxidant system and photosynthesis of cucumber seedlings under calcium nitrate stress were studied using “Jinchun No.2” cucumber as test material. The results showed that compared with the control, the calcium nitrate stress resulted in the generation of superoxide anion, the content of hydrogen peroxide and malondialdehyde and the membrane permeability of cucumber seedling leaves. The net photosynthetic rate, stomatal conductance, transpiration rate, The CO2 concentration decreased by 33.39%, 55.18%, 30.23% and 34.56%, respectively. The dry matter accumulation of seedlings decreased by 44.17% and the growth was significantly inhibited. However, putrescine increased the activities of superoxide dismutase (SOD) and peroxidase POD and CAT, and decreased the production of superoxide anion, the content of hydrogen peroxide and malondialdehyde (MDA) and the permeability of cell membrane, alleviated the decline of Pn under the stress of calcium nitrate, The accumulation of dry matter in seedlings increased by 21.59%, which accelerated the growth. It can be seen that putrescine can reduce the lipid peroxidation of cucumber seedlings by regulating the anti-oxidative activity of cucumber seedlings under the calcium nitrate stress, thus maintaining its high photosynthetic capacity and effectively promoting the growth of calcium nitrate Cucumber seedling growth under stress.