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The Rayleigh-Taylor instability in two-dimensional incompressible fluids at arbitrary Atwood numbers is studied by expanding the perturbation velocity potential to third order. The second and third harmonic generation effects of single-mode perturbation are analyzed, as well as the nonlinear correction to the exponential growth of the fundamental modulation. The mode coupling coefficients are dependent on the Atwood numbers. Our simulations support the weakly nonlinear results. We find that the ratio of the nonlinear saturation amplitude ηs and the perturbation wavelength λ is dependent on the Atwood number AT and the relation is ηs/λ=(1/π)[√2/5/√(1+3AT2 )].