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目的观察视网膜色素上皮(RPE)细胞中,IL-6的表达及地塞米松对IL-6表达的抑制作用。方法培养的人RPE经IL-1β(10μg/L)刺激及地塞米松作用后,采用ELASA、免疫组化和原位杂交等方法,检测培养的人RPEIL-6mRNA及其蛋白的表达。结果用IL-1β刺激后8h,RPE细胞表达的IL-6约为2000ng/L·106细胞;地塞米松可明显抑制IL-6蛋白的产生(200ng/L·106细胞),与对照组相比较差异显著(P∨0.01)。免疫组化染色显示,表达的IL-6在RPE细胞胞浆中呈浓淡不一的棕黄色,地塞米松作用组染色较浅。原位杂交表明,IL-6mRNA在RPE细胞胞浆中的表达呈浓淡不一的紫蓝色,地塞米松作用组染色与对照组相同。图像分析显示,二者的吸光度(A)值无明显差别(P∧0.05)。结论在IL-1β刺激下,人RPE细胞可表达IL-6的mRNA及其蛋白,地塞米松能有效地抑制RPE细胞中IL-6蛋白的表达。
Objective To observe the expression of IL-6 in retinal pigment epithelium (RPE) cells and the inhibitory effect of dexamethasone on the expression of IL-6. Methods Human RPE cultured in RPEIL-6 and its protein were detected by ELASA, immunohistochemistry and in situ hybridization after stimulation with IL-1β (10μg / L) and dexamethasone. Results IL-6 expression in RPE cells was about 2000 ng / L · 106 cells 8 h after stimulation with IL-1β. Dexamethasone could significantly inhibit the production of IL-6 protein (200 ng / L · 106 cells) The difference was significant (P ∨ 0.01). Immunohistochemical staining showed that the expression of IL-6 in the cytoplasm of RPE cells was shades of brown, dexamethasone effect group staining lighter. In situ hybridization showed that the expression of IL-6mRNA in the cytoplasm of RPE cells was purple and purple, while the dexamethasone group was the same as the control group. Image analysis showed no significant difference in absorbance (A) between the two (P 0 0.05). CONCLUSION: Human RPE cells can express IL-6 mRNA and protein under the stimulation of IL-1β. Dexamethasone can effectively inhibit the expression of IL-6 protein in RPE cells.