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Kanamycin was used to screen T0 seeds of the variety Dongnong 46 transformed by means of pollen-tube method. The results showed that 400 mg·L-1 kanamycin could inhibit growth of non-transgenic plants, and 2 positive plants were gotten combined with Gus dyeing and PCR detection. It is proved that this method is e-conomic and effective in preliminary screening the transgenic plants.
Kanamycin was used to screen T0 seeds of the variety Dongnong 46 transformed by means of pollen-tube method. The results showed that 400 mg · L-1 kanamycin could inhibit growth of non-transgenic plants, and 2 positive plants were gotten combined with Gus dyeing and PCR detection. It is verified that this method is e-conomic and effective in preliminary screening the transgenic plants.