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【目的】对顶果木灰霉病病原菌进行鉴定,以明确其分类地位,为顶果木灰霉病的防控提供参考依据。【方法】从广西南宁市良凤江国家森林公园苗圃基地采集顶果木灰霉病病害标本,对病害进行症状描述、组织分离和致病性测定,观察致病菌的形态学特征并进行r DNA-ITS序列分析。【结果】分离获得顶果木灰霉病致病菌代表菌株DGM5-6。DGM5-6菌株在PDA上长势良好,毛绒状菌落初期呈淡黄白色,后期逐渐变为灰白色;气生菌丝呈放射状生长,开始为白色,生长后期为灰色;病菌分生孢子梗单生,灰色至褐色,分枝,顶端膨大成球形,其上有多个小梗,梗上聚生卵形、圆形或椭圆形的分生孢子;分生孢子呈浅灰色至无色,单胞,大小为10.0~12.5μm×7.5~10.0μm。DGM5-6菌株ITS基因扩增片段长度为535 bp,其r DNA-ITS序列与Gen Bank中灰葡萄孢(Botrytis cinerea Pers.ex Fr)序列的同源性达99%。【结论】引起广西顶果木灰霉病的病原为灰葡萄孢(Botrytis cinerea Pers.ex Fr),该病原菌寄主范围广,应结合苗圃清园、圃地治理、合理密植、生物防治和化学防治等进行防控。
【Objective】 The objective of this study was to identify the pathogen of Botrytis cinerea so as to clarify its taxonomic status and provide reference for the prevention and control of Botrytis cinerea. 【Method】 The gray mold disease of top wood was collected from the nursery of Liangfengjiang National Forest Park in Nanning, Guangxi. The disease symptoms were described, the tissues were separated and the pathogenicity was determined. Morphological characteristics of pathogenic bacteria were observed and rDNA -ITS sequence analysis. 【Result】 The representative strain DGM5-6 was isolated from Botrytis cinerea. The DGM5-6 strain grew well on the PDA, the early fluffy colonies were pale yellow and white, the latter gradually turned gray; aerial mycelium showed radial growth, beginning to white, gray at the late growth stage; , Gray to brown, branched, apical dilated into a sphere with multiple small stems on it, polygamous ovoid, round or oval conidia; conidia pale gray to colorless, , The size is 10.0 to 12.5 μm × 7.5 to 10.0 μm. The amplified ITS fragment of DGM5-6 strain was 535 bp in length, and its sequence homology with the nucleotide sequence of Botrytis cinerea Pers.ex Fr in GenBank was 99%. 【Conclusion】 The pathogen causing Botrytis cinerea in Guangxi was Botrytis cinerea Pers.ex Fr, which had a wide host range and should be combined with nursery clearing garden, nursery land management, close planting density, biological control and chemical control For prevention and control.