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构建了一种检测双酚A(BPA)的电化学适配体传感器。利用在线电化学方法将氧化石墨烯还原为石墨烯,通过石墨烯与单链DNA之间的相互作用,将BPA适配体单链DNA吸附固定在修饰电极上,制备了BPA电化学适配体传感器。以铁氰化钾-亚铁氰化钾平衡电对为电化学探针,利用电化学循环伏安法和差分脉冲伏安法对BPA传感器的性能进行了研究。结果表明,在最优化实验条件下,传感器对BPA的检测线性范围在1.0×10~(-15)~1.0×10~(-10)mol/L之间,检出限为3.3×10~(-16)mol/L(S/N=3)。
An electrochemical aptamer sensor was developed to detect bisphenol A (BPA). An online electrochemical method was used to reduce graphene oxide into graphene. The BPA aptamer single-stranded DNA was adsorbed and immobilized on the modified electrode through the interaction between graphene and single-stranded DNA. The BPA electrochemical aptamer sensor. The electrochemical probes were prepared by the equilibrium electrovaporization of potassium ferricyanide - potassium ferrocyanide. The electrochemical performance of BPA sensors was studied by electrochemical cyclic voltammetry and differential pulse voltammetry. The results showed that the linear range of the sensor for BPA detection ranged from 1.0 × 10 ~ (-15) to 1.0 × 10 ~ (-10) mol / L with the detection limit of 3.3 × 10 ~ -16) mol / L (S / N = 3).