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目的:建立磐安产延胡索的HPLC指纹图谱鉴别方法,比较遗传变异的大叶型和小叶型延胡索之间的区别。方法:以延胡索乙素为参照物,采用HPLC检测方法:色谱条件:Ultimate LP-C18色谱柱(4.6mm×250mm,5μm),乙腈-三乙胺乙酸溶液(每1000mL水中加冰乙酸30mL、三乙胺8mL)二元梯度洗脱,体积流量0.8mL/min,检测波长280nm,进样量20μL,柱温35℃。结果:确定了11个共有峰,建立了延胡索的HPLC指纹图谱,比较研究了大、小叶型延胡索不同加工方法的样品,结果相似度均在0.90以上。结论:本法操作简便,重现性好,可有效控制延胡索的质量,并为产地优质品种选育提纯及加工提供实验依据。
OBJECTIVE: To establish HPLC fingerprinting identification method of Pan-Yan-Corydalis Rhizoma and to compare the difference between the large-leaf-type and the leaflet-type Corydalis of genetic variation. Methods: The HPLC method was used for the determination of tetrahydropalmatine. The chromatographic conditions were: Ultimate LP-C18 column (4.6mm × 250mm, 5μm), acetonitrile-triethylamine in acetic acid solution Ethylamine 8mL) binary gradient elution volume flow 0.8mL / min, detection wavelength 280nm, injection volume 20μL, column temperature 35 ℃. Results: Eleven common peaks were identified and the HPLC fingerprints of Corydalis yanhusuo were established. The samples with different processing methods of large and small leaf corydalis were compared. The similarity of the results was above 0.90. Conclusion: This method is simple, reproducible, and can effectively control the quality of Corydalis yanhusuo, and provide experimental basis for selection, purification and processing of high quality varieties in the producing areas.