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目的:建立降脂宁有效部位中金丝桃苷和异槲皮苷的HPLC含量测定方法。方法:采用SunFire C18色谱柱(150 mm×4.6 mm,5μm),柱温35℃,流动相为乙腈-0.01%甲酸水溶液(15∶85,v/v),流速为0.8 mL.min-1,检测波长354 nm。结果:金丝桃苷、异槲皮苷平均回收率分别为100.0%和99.8%,RSD分别为2.6%和2.5%。结论:3批样品测定结果表明,该方法简便、准确,可用于降脂宁有效部位中金丝桃苷和异槲皮苷的含量测定。
OBJECTIVE: To establish a HPLC method for the determination of hyperoside and isoquercitrin in effective fractions of Jiangzhining. METHODS: SunFire C18 column (150 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile-0.01% formic acid aqueous solution (15:85, v / v) at a flow rate of 0.8 mL · min- Detection wavelength of 354 nm. Results: The average recoveries of hyperoside and isoquercetin were 100.0% and 99.8%, respectively. The RSDs were 2.6% and 2.5% respectively. Conclusion: The results of three batches of samples show that the method is simple and accurate, and can be used to determine the content of hyperoside and isoquercitrin in effective fractions of Jiangzhining.