采用外周血白细胞促凝血活性（LPCA）试验，检测正常排卵周期生育妇女，不明原因的继发闭经和不孕患者对卵巢抗原的免疫反应。结果表明，待测样本对卵巢抗原的细胞免疫反应增强，继发闭经和不孕患者的LPCA阳性率分别为65．0％和31．9％，明显高于对照组（P＜0．01），且LPCA反应与血清抗卵巢抗体之间有非常显著的关联性（P＜0．01）．提示，闭经与不孕患者对卵巢抗原产生的细胞免疫、体液免疫反应出现的机会存在高度一致． The peripheral blood leukocyte procoagulant activity (LPCA) test was used to detect the immune response to ovarian antigens in women of normal ovulation cycle and unexplained secondary amenorrhea and infertility. The results showed that the cellular immune response to ovarian antigen in the sample was enhanced, and the positive rates of LPCA in secondary amenorrhea and infertility patients were 65.0% and 31.9%, respectively, which were significantly higher than those in the control group (P <0.01) , And there was a significant correlation between LPCA reaction and serum anti-ovarian antibody (P <0.01). Tip, amenorrhea and infertility patients on ovarian antigen-generated cellular immunity, there is a highly consistent opportunity for humoral immune response.