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镉是一种非必需的重金属元素,对动植物有严重毒害作用。几个与ABC1(activity of the bc1 complex)家族有关的基因参与植物镉胁迫的应答。本研究从玉米中克隆并鉴定了一个类ABC1基因,命名为ZmABC1-10。该基因cDNA全长2 519 bp,包含一个2 250 bp的开放阅读框,编码一个预测的叶绿体膜蛋白。启动子顺式元件扫描发现该基因含有大量的非生物胁迫、光以及植物激素应答元件。表达模式分析表明,该基因主要在叶片、茎秆等绿色组织中表达。镉处理实验表明,该基因能够被诱导并且受植物发育时期的调控。除镉之外,该基因还受多种非生物因素包括ABA、H2O2、干旱和黑暗的共同调控。此外,本研究利用基因组序列信息共鉴定出19个玉米ABC1基因。对植物界8个代表性物种中148个ABC1蛋白进行系统发育分析表明,在长期进化过程中植物ABC1蛋白已经发生了分化;物种特异性扩张是植物中该家族进化的主要动力。这些结果表明ZmAbc1-10是一个镉应答因子并且可能在植物对非生物胁迫的适应中发挥重要作用。
Cadmium is a non-essential heavy metal element that has serious toxic effects on plants and animals. Several genes involved in ABC1 (activity of the bc1 complex) family are involved in plant Cd stress response. In this study, an ABC1-like gene was cloned and identified from maize and named ZmABC1-10. The gene cDNA is 2 519 bp in length and contains a 2 250 bp open reading frame encoding a predicted chloroplast membrane protein. The promoter cis-element scan found that the gene contains a large number of abiotic stress, light and plant hormone response elements. Expression pattern analysis showed that the gene was mainly expressed in green tissues such as leaves and stems. Cadmium treatment experiments show that the gene can be induced and regulated by the plant development period. In addition to cadmium, the gene is also regulated by a variety of abiotic factors including ABA, H2O2, drought and darkness. In addition, 19 maize ABC1 genes were identified using genomic sequence information. Phylogenetic analysis of 148 ABC1 proteins from 8 representative species in the plant kingdom indicated that plant ABC1 protein had been differentiated during long-term evolution. Species-specific expansion was the main motivation for this family evolution in plants. These results indicate that ZmAbcl-10 is a cadmium response factor and may play an important role in the adaptation of plants to abiotic stresses.