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目的乙型肝炎病毒（ＨＢＶ）是原发性肝细胞肝癌（ＨＣＣ）的主要诱导因素之一。为探讨ＨＢＶ在ＨＣＣ中的重要性，我们检测了ＨＣＣ患者癌组织及血清中的ＨＢＶ前Ｃ区基因。方法根据ＨＢＶ前Ｃ区ＤＮＡ序列，采用聚合酶链式反应（ＰＣＲ）对２３例手术切除后病理确诊有ＨＣＣ患者癌组织以及血清进行了检测。结果ＰＣＲ显示２３例ＨＣＣ患者癌组织和血清中ＨＢＶ前Ｃ区基因的阳性率分别为５２．２％（１２／２３）和３０．４％（７／２３）。结论ＨＢＶ前Ｃ区基因广泛存在于ＨＣＣ组织中，其中有２１．８％的ＨＣＣ患者的血清ＨＢＶ前Ｃ区基因为阴性而癌组织中为阳性，提示ＨＢＶ前Ｃ区基因在肝细胞中可能存在整合。
Objective Hepatitis B virus (HBV) is one of the major inducing factors of primary hepatocellular carcinoma (HCC). To investigate the importance of HBV in HCC, we tested the HBV pre-C gene in cancer tissues and serum of HCC patients. Methods Based on the DNA sequence of HBV pre-C region, 23 cases of pathologically confirmed HCC cancer tissue and serum were detected by polymerase chain reaction (PCR). Results PCR showed that the positive rates of HBV pre-C genes in cancer tissues and serum of 23 HCC patients were 52.2% (12/23) and 30.4% (7/23), respectively. Conclusion The HBV pre-C gene is widely found in HCC tissues. Among them, 21.8% of HCC patients have negative serum HBV pre-C region and positive in cancer tissue, suggesting that HBV pre-C gene may exist in hepatocytes. Integration.