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磷脂酶A_2(PLA_2;EC3.1.4)作用于磷脂的2-酰基位置,使磷脂主要释放出顺不饱和脂肪酸和溶血磷脂,此酶在膜磷脂的转换、外来物(如细菌和病毒)的消化和二十碳四烯酸的释放与二十酸的合成中起重要作用。作者以[1~(14)C]油酸制剂标记高压灭菌的大肠埃希氏杆菌(E.coli)作为基质,测定了正常人和尿毒症血浆中PLA_2的活性。结果显示,正常人血浆未稀释、稀释10倍和25倍PLA_2活性(umol/L/分钟)X±SD分别为0.25(0.04)、0.53(0.13)和0.73(0.28);尿毒症病人血浆分别为1.97(0.778)、5.03(2.35)和8.44(4.11)。在未稀释的血浆中PLA_2活性尿毒症比正常人大8倍,稀释血浆两组试验者
Phospholipase A2 (PLA2; EC3.1.4) acts on the 2-acyl position of the phospholipid, causing the phospholipid to release predominantly unsaturated fatty acids and lysophospholipids, which are involved in membrane phospholipid conversion, digestion of foreign objects such as bacteria and viruses And arachidonic acid release and icosanoic acid synthesis plays an important role. The authors used autoclaved E. coli labeled with [1 ~ (14) C] oleic acid as matrix to determine PLA 2 activity in normal human and uremic plasma. The results showed that the plasma of normal people was 0.25 (0.04), 0.53 (0.13) and 0.73 (0.28), respectively. The plasma of patients with uremia was 1.97 (0.778), 5.03 (2.35) and 8.44 (4.11). In undiluted plasma PLA 2 activity uremia eight times larger than normal, diluted plasma two groups of test subjects