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目的：观察乙型肝炎病毒（ＨＢＶ）ａｙｗ亚型全基因组转基因小鼠的肝、肾等组织的病理改变。方法：选取２４只１３～２５周龄的清洁级ＩＣＲ品系ＨＢＶ转基因小鼠及１５只正常ＩＣＲ小鼠为对照进行病理大体观察，并重点取肝、肾、脾、肺、心、脑、睾丸（卵巢）、唾液腺、肌肉和皮肤等组织，切片ＨＥ染色后进行光镜和透射电镜形态观察。结果：上述两种清洁级小鼠大体和光镜下各器官和组织未见有明显病变；两种小鼠移入普通级环境下饲养４周后肝脏等器官发生机会性感染的机会相似；透射电镜下所有被检转基因小鼠的部分肝细胞扩张的滑面内质网等处有ＨＢｓＡｇ样颗粒和Ｄａｎｅ样颗粒，部分转基因小鼠之肾小管上皮细胞内见类似病毒颗粒；肝、肾细胞内未见其他亚微病理损害。结论：所建成的ＨＢＶ转基因小鼠既能在肝（其次肾）细胞内产生ＨＢＶ样病毒颗粒，但又不产生明显病理损害。
Objective: To observe the pathological changes in the liver, kidney and other tissues of hepatitis A virus (HBV) ayw whole genome transgenic mice. Methods: Twenty-four clean-grade ICR HBV-transgenic mice and 15 normal ICR mice of 13 to 25 weeks old were selected for pathological observation. Liver, kidney, spleen, lung, heart, brain and testis Ovary), salivary gland, muscle and skin. The sections were stained with HE and observed with light microscope and transmission electron microscope. Results: There were no obvious pathological changes in the organs and tissues of the above two clean mice in general and light microscope. The chances of opportunistic infections of liver and other organs were similar after the two mice were fed for 4 weeks in ordinary environment. The transmission electron microscope HBsAg-like particles and Dane-like particles were found in the sliding endoplasmic reticulum of some hepatocytes in all the transgenic mice that were tested. Similar virus particles were found in the renal tubular epithelial cells of some transgenic mice. No hepatic and renal cells Other sub-micro pathological damage. Conclusion: The constructed HBV transgenic mice both produce HBV-like virus particles in the liver (secondarily kidney) cells without significant pathological damage.