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以不同浓度的 N-乙酰基 - D-葡萄糖胺溶液在波长 2 0 0 ,2 0 1 ,2 0 2 ,2 0 3nm处的吸光度值对波长作图得到线性关系良好的 1组直线 ,其斜率与对应溶液的浓度成正比 ,线性范围为 0 .0 0 2 5～0 .0 4 0 0 mg/m L,回归方程为 κ=- 2 .2 52 c- 0 .0 0 2 7,相关系数 r=0 .99995(n=6)。不同批号壳聚糖样品测定结果的相对标准偏差 0 .2 0 %～ 0 .52 % (n=5) ,回收率在 1 0 0 .2 %～ 1 0 1 .6%之间。本法解决了单波长紫外分光光度法中共存物吸收干扰问题 ,并简化了紫外一阶导数法中导数值的求法。实验结果表明 ,该法测定壳聚糖的脱乙酰度准确度高 ,重现性好 ,试样无须特殊处理 ,适用于可溶性壳聚糖脱乙酰度的测定。
A series of straight lines with good linearity were obtained by plotting the absorbance at different wavelengths of N-acetyl-D-glucosamine at wavelengths of 200, 210, 202 and 203 nm with the slope And the concentration of the corresponding solution is proportional to the linear range of 0.002 5 ~ 0.400 mg / m L, the regression equation was κ = - 2. 52 c-0. 0 0 2 7, the correlation coefficient r = 0 .99995 (n = 6). The relative standard deviations (RSDs) of different chitosan samples from 0.2% to 0.52% (n = 5) were obtained. The recoveries ranged from 100% to 120.1%. The method solves the problem of interference of the co-deposit absorption in the single-wavelength ultraviolet spectrophotometry and simplifies the calculation of the derivative value in the first-derivative UV method. The experimental results show that the determination of chitosan deacetylation degree with high accuracy, good reproducibility, the sample without special treatment, suitable for the determination of the degree of deacetylation of soluble chitosan.