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目的:探讨三生饮对大鼠局灶性脑缺血后海马区细胞凋亡相关基因Bc l-2和Bax蛋白表达的影响。方法:用线栓法建立大鼠局灶性脑缺血再灌注模型,用SP免疫组织化学方法,检测再灌注后3、6、12、24 h及3 d不同时段海马区Bc l-2和Bax免疫反应阳性细胞平均计数,统计分析三生饮治疗组和模型组的表达量。结果:两组海马区神经元均大量表达Bax蛋白,两组间无统计学差异;与模型组相比,三生饮治疗组大鼠缺血侧海马区Bc l-2蛋白表达增多,表达时程延长(P<0.05);在缺血侧海马不同区域,Bc l-2/Bax之值不同。结论:大鼠脑缺血再灌注后海马区神经元均有Bc l-2和Bax蛋白的表达,而灌给三生饮可促进海马区Bc l-2阳性细胞的表达,提高Bc l-2/Bax值;但三生饮治疗组大鼠海马区脑细胞仍然过度表达Bax阳性蛋白,最终发生凋亡。
Objective: To investigate the effect of Sanshengyin on the expression of apoptosis-related genes Bcl-2 and Bax in hippocampus after focal cerebral ischemia in rats. METHODS: The model of focal cerebral ischemia and reperfusion in rats was established by thread embolism method. SP immunohistochemical method was used to detect Bc l-2 and hippocampus at 3, 6, 12, 24, and 3 days after reperfusion. The average number of Bax immunoreactive positive cells was counted, and the expression levels of the three raw drink treatment groups and the model group were statistically analyzed. RESULTS: The expression of Bax protein was significantly increased in hippocampal neurons in both groups. There was no statistical difference between the two groups. Compared with the model group, Bcl-2 protein expression was increased in the ischemic hippocampus of the Sanshengyin treatment group. Duration was prolonged (P<0.05); Bcl-2/Bax values were different in different areas of the hippocampus on the ischemic side. Conclusion: The expression of Bcl-2 and Bax proteins in neurons of hippocampus after cerebral ischemia-reperfusion in rats is increased. The administration of Sanshengyin can increase the expression of Bcl-2 positive cells in hippocampus and increase the expression of Bcl-2. /Bax value; However, the brain cells in the hippocampus of the Sanshengyin treatment group still overexpressed Bax-positive protein, and eventually apoptosis occurred.