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目的观察金钗石斛生物总碱(DNLA)对Wistar大鼠高脂血症和肝脏脂肪变性的影响。方法将受试雄性Wister大鼠48只随机平均分为6组,分别为正常对照组,模型组,辛伐他汀组和DNLA 40、80、160 mg·kg~(-1)组。高脂饲养建立高脂血症大鼠模型,同时采用灌胃给药。16wk后采用酶法测定血脂,进行肝脏组织HE染色,观察DNLA对高脂血症大鼠模型血脂和肝脏脂肪变性的影响。结果和模型组比较,DNLA各剂量组高脂血症大鼠血清脂质含量无明显变化,但DNLA 40、80和160mg·kg~(-1)能够降低高脂血症大鼠肝脏组织丙二醛含量和升高超氧化物歧化酶活性(P<0.05),使高脂血症大鼠肝脏脂肪变性明显减轻。结论 DNLA能够减轻高脂血症大鼠肝脏组织的脂肪变性,其作用机制可能与抗氧化损伤有关。
Objective To observe the effects of DNLA on hyperlipidemia and hepatic steatosis in Wistar rats. Methods Forty-eight male Wistar rats were randomly divided into 6 groups: normal control group, model group, simvastatin group and DNLA 40,80,160 mg · kg -1 group. High fat diet to establish hyperlipidemic rat model, while using gavage. After 16 weeks, the blood lipids were measured by enzymatic method and the liver tissues were stained with HE to observe the effect of DNLA on the lipids and hepatic steatosis in hyperlipidemic rats. Results Compared with the model group, there was no significant change in serum lipids in hyperlipidemic rats treated with DNLA, but DNLA 40, 80 and 160 mg · kg -1 could reduce the level of malondialdehyde Aldehyde content and superoxide dismutase activity (P <0.05), so that the fatty degeneration of liver in hyperlipidemic rats was significantly reduced. Conclusion DNLA can reduce the fatty degeneration of liver tissue in hyperlipidemic rats, which may be related to the anti-oxidative damage.