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目的:建立HPLC测定绞股蓝中槲皮素含量的方法。方法:采用Ultrasphere C_(18)(250 mm×4.6 mm,5μm);流动相为甲醇-0.4%磷酸(50:50);流速:1.0 ml·min~(-1)。检测波长为360 nm。结果:槲皮素在0.154～0.770μg范围内与峰面积具有良好的线性关系,r=0.999 9;加样回收率为98.17%,RSD为0.88%(n=6)。结论:该法简便、准确,灵敏度高,重复性好,可作为控制绞股蓝药材中黄酮类成分的质控方法之一。
Objective: To establish a HPLC method for the determination of quercetin in Gynostemma pentaphyllum. Methods: Ultrasphere C 18 (250 mm × 4.6 mm, 5 μm) was used. The mobile phase was methanol-0.4% phosphoric acid (50:50). The flow rate was 1.0 ml · min -1. The detection wavelength is 360 nm. Results: Quercetin had a good linear relationship with the peak area in the range of 0.154 ~ 0.770 μg, r = 0.999 9; the recoveries were 98.17% and the RSD was 0.88% (n = 6). Conclusion: The method is simple, accurate, sensitive and reproducible. It can be used as one of the quality control methods to control flavonoids in Gynostemma.