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目的探讨苦参碱对人急性红白血病细胞株TF-1 SALL4基因及Wnt/β-catenin信号通路下游靶基因表达的影响。方法用不同浓度的苦参碱(0.5、1.0、2.0 g/L)处理TF-1细胞,另设对照组(不加苦参碱)。苦参碱作用48 h后,采用实时荧光定量PCR法检测各组TF-1细胞中SALL4基因及Wnt/β-catenin信号通路下游靶基因β-catenin、C-myc、Cyclin DI的表达水平,并分析SALL4基因与β-catenin、C-myc及Cyclin DI表达的相关性;Western blot法检测各组TF-1细胞中SALL4蛋白的表达水平。结果经不同浓度苦参碱处理48 h后,TF-1细胞中SALL4、β-catenin、C-myc、Cyclin DI基因的表达水平及SALL4蛋白的表达水平与对照组相比,均明显下降,且呈浓度依赖性(P<0.05);SALL4基因与β-catenin、C-myc、Cyclin DI基因的表达明显相关(r s值分别为0.912、0.818和0.832,P均<0.01)。结论苦参碱对TF-1细胞中SALL4基因和蛋白表达及Wnt/β-catenin信号通路下游靶基因β-catenin、C-myc、Cyclin DI的抑制可能在抑制细胞增殖、诱导细胞凋亡过程中起重要作用。
Objective To investigate the effect of matrine on the expression of SALL4 and Wnt / β-catenin signaling in human acute erythroleukemia cell line. Methods TF-1 cells were treated with different concentrations of matrine (0.5, 1.0 and 2.0 g / L), and another control group (without matrine). After 48 hours of matrine treatment, the expression of SALL4 and Wnt / β-catenin signaling pathway downstream target genes β-catenin, C-myc and Cyclin DI were detected by real-time fluorescence quantitative PCR The correlation between SALL4 gene expression and β-catenin, C-myc and Cyclin DI expression was analyzed. The expression of SALL4 protein in TF-1 cells was detected by Western blot. Results Compared with the control group, the expression levels of SALL4, β-catenin, C-myc and Cyclin DI and the expression of SALL4 protein in TF-1 cells decreased significantly after treatment with different concentrations of matrine for 48 h (P <0.05). The expression of SALL4 was significantly correlated with the expression of β-catenin, C-myc and Cyclin DI (rs = 0.912,0.818 and 0.832, respectively, P <0.01). Conclusion Matrine inhibits the expression of SALL4 gene and protein and the downstream targets of β-catenin, C-myc and Cyclin DI in Wnt / β-catenin signaling pathway in TF-1 cells, which may be involved in the inhibition of cell proliferation and apoptosis induction makes an important impact.