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目的:研究蛇葡萄素在体内外对肿瘤生长的抑制作用。方法:用MTT法测定蛇葡萄素对人鼻咽癌HK-1细胞和人乳腺癌MCF-7细胞的体外细胞毒实验;观察蛇葡萄素对C57BL/6小鼠移植性B16黑色素瘤的体内抑瘤作用;用流式细胞仪测定含药小鼠血清对B16细胞周期及细胞增殖的影响。结果:蛇葡萄素对人鼻咽癌HK-1细胞及人乳腺癌MCF-7细胞的IC50分别为50.0μg/ml及79.4μg/ml。在100mg/kg、150mg/kg及200mg/kg的剂量下,与生理盐水对照组比较,蛇葡萄素对C57BL/6小鼠移植性B16黑色素瘤的抑瘤率分别为25.54%(P<0.05)、32.03%(P<0.01)及54.55%(P<0.001);60mg/kg氮烯咪胺(阳性对照组)的抑瘤率为59.31%,与200mg/kg的药物组比较无显著性差异(P>0.05);在150mg/kg及200mg/kg蛇葡萄素腹腔给药后10min的小鼠含药血清的作用下,B16细胞的G1期和G2/M期细胞数增高,S期细胞数减少,分裂增殖指数分别降低19.1%及21.7%。结论:蛇葡萄素对体外肿瘤细胞HK-1和MCF-7的增殖,及体内小鼠移植性B16黑色素瘤的生长均具有显著的抑制作用。
Objective: To study the inhibitory effect of schizontidine on tumor growth in vitro and in vivo. METHODS: MTT assay was used to determine the in vitro cytotoxicity effect of arachidin on human nasopharyngeal carcinoma HK-1 cells and human breast cancer MCF-7 cells; and the inhibitory effect of arachidin on C57BL/6 mice transplanted B16 melanoma in vivo was observed. Tumor effect; flow cytometry was used to determine the effect of drug-containing mouse serum on B16 cell cycle and cell proliferation. RESULTS: The IC50 values of arbustin on human nasopharyngeal carcinoma HK-1 cells and human breast cancer MCF-7 cells were 50.0 μg/ml and 79.4 μg/ml, respectively. At the doses of 100 mg/kg, 150 mg/kg and 200 mg/kg, the inhibition rate of anistratin on C57BL/6 mice transplanted B16 melanoma was 25.54% compared with the saline control group (P<0.05). , 32.03% (P<0.01) and 54.55% (P<0.001); 60mg/kg nalcipamide (positive control group) tumor inhibition rate was 59.31%, and there was no significant difference compared with 200mg/kg drug group ( P>0.05); Under the effect of mouse serum containing drug 10 min after 150 mg/kg and 200 mg/kg aniscarin administration, the number of cells in G1 and G2/M phases of B16 cells increased, and the number of cells in S phase decreased. The division proliferation index decreased by 19.1% and 21.7%, respectively. CONCLUSION: Ampullins can significantly inhibit the proliferation of tumor cells HK-1 and MCF-7 in vitro and the growth of transplanted B16 melanoma in mice.