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A plasmid expressing antisense MDRl cDNAsegment was introduced into KB_(v200) which inductedmultiple drugs to VCT (vincristine, 175 fold-resistancehigher than that in original KB cells) and ADM(adriamycin, 14. 5 fold) resulting over-expression ofMDRl. We used the primers for antisense RNA asfollowing: upstream 5′OGAATTCTGAAACCTGTAAGCAGCAACC 3′: downstream 5′CGGGATCCTCGA
A plasmid expressing antisense MDR1 cDNA segment was introduced into KB_(v200) which inducted multiple drugs to VCT (vincristine, 175 fold-resistancehigher than that in original KB cells) and ADM(adriamycin, 14. 5 fold) resulting over-expression ofMDRl. We used The primers for antisense RNA asfollowing: upstream 5′OGAATTCTGAAACCTGTAAGCAGCAACC 3′: downstream 5′CGGGATCCTCGA