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目的筛选肝细胞癌（ＨＣＣ）相关基因，探讨其在ＨＣＣ发病中的临床意义。方法用差异显示技术对比研究正常肝组织、ＨＣＣ组织及癌旁肝组织之间ｍＲＮＡ的表达差异，以肝癌ｃＤＮＡ片段ＭＲＧ９８．２为探针，对１０例ＨＣＣ及癌旁肝组织进行斑点印渍分析，并通过逆转录聚合酶链反应（ＲＴ－ＰＣＲ）方法检测这些标本血管内皮生长因子（ＶＥＧＦ）ｍＲＮＡ的表达水平。结果从ＨＣＣ组织中获得的差示片段ＭＲＧ９８．２在７例ＨＣＣ标本中表达阳性（７０％），癌旁肝组织弱表达（２／１０）或无表达。 ＶＥＧＦ ｍＲＮＡ在７例ＭＲＧ９８．２阳性表达的ＨＣＣ组织中６例表达上调。结论 ＭＲＧ９８．２是ＨＣＣ相关的基因片段，其表达与ＶＥＧＦ ｍＲＮＡ相关，并可能与肿瘤浸润转移、患者的预后不良有关。
Objective To screen the related genes of hepatocellular carcinoma (HCC) and discuss its clinical significance in the pathogenesis of HCC. Methods Differential display technique was used to compare the expression of mRNA between normal liver tissue, HCC tissue and adjacent liver tissues. The liver cancer cDNA fragment MRG98.2 was used as a probe to perform spot blot analysis on 10 cases of HCC and adjacent liver tissues. The expression level of vascular endothelial growth factor (VEGF) mRNA in these specimens was detected by reverse transcription polymerase chain reaction (RT-PCR). Results The differential segment MRG98.2 obtained from HCC tissue was positive in 7 HCC specimens (70%), and the para-carcinoma tissue was weakly expressed (2/10) or not. VEGF mRNA was up-regulated in 6 of 7 HCC tissues positively expressed by MRG98.2. Conclusion MRG98.2 is a gene fragment associated with HCC. Its expression is related to VEGF mRNA and may be related to tumor invasion and metastasis and poor prognosis of patients.