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目的研究热休克蛋白27相关蛋白1(heat shock 27 000 associated protein 1,HSPBAP1, GenBank:AK096705)在耐药性癫痫患者脑组织中的表达,探讨其在耐药性癫痫发病机制中的作用。方法从重庆医科大学附属第一医院神经内科建立的耐药性癫痫脑库中随机抽取36例耐药性癫痫患者术后脑组织,用荧光定量聚合酶链反应(FQ-PCR)和免疫组化法对HSPBAP1的表达进行检测,并与8例对照进行比较。结果耐药性癫痫患者脑组织中HSPBAP1 mRNA的相对表达量是对照组的34．11倍;HSPBAP1蛋白在耐药性癫痫患者颞叶(0．0507±0．0003,P<0．01)和海马(0．0488±0．0196,P<0．05)中的表达量,与对照组相同部位比较明显增加。结论耐药性癫痫患者脑组织中HSPBAP1表达增高,能抑制热休克蛋白27(HSP27)的神经元保护作用,可能是导致神经元凋亡及海马苔藓纤维发芽的一个重要原因。
Objective To study the expression of heat shock 27 000 associated protein 1 (HSPBAP1, GenBank: AK096705) in brain tissue of patients with drug-resistant epilepsy and to explore its role in the pathogenesis of drug-resistant epilepsy. Methods Thirty-six patients with drug-resistant epilepsy were randomly selected from the brain bank of drug-resistant epilepsy established in the First Affiliated Hospital of Chongqing Medical University. Fluorescent quantitative polymerase chain reaction (FQ-PCR) and immunohistochemistry Method HSPBAP1 expression was detected and compared with 8 cases. Results The relative expression level of HSPBAP1 mRNA in brain tissue of patients with drug-resistant epilepsy was 34.11 times that of the control group. HSPBAP1 protein expression was significantly increased in the temporal lobe of patients with drug-resistant epilepsy (0.0507 ± 0.0003, P <0.01) The expression level in hippocampus (0.0488 ± 0.0196, P <0.05) was significantly higher than that in the control group. Conclusions Increased expression of HSPBAP1 in the brain tissue of patients with drug-resistant epilepsy can inhibit the neuroprotective effect of heat shock protein 27 (HSP27), which may be one of the important causes of neuron apoptosis and germination of mossy fiber in hippocampus.