论文部分内容阅读
目的:考查柱后衍生条件对伏格列波糖峰柱后衍生化HPLC检测的影响,为伏格列波糖检测条件的优化提供依据。方法:考察不同反应浴温度、冷却浴温度、衍生试剂浓度及流动相流速/衍生试剂流速比例等因素对伏格列波糖柱后衍生化检测的影响。结果:在实验考察条件下,伏格列波糖峰面积随着反应浴温度的升高而增大;在一定范围内,衍生试剂浓度的增大导致伏格列波糖的峰面积增加,继续增大衍生试剂的浓度,峰面积反而减小;随着流动相流速/衍生试剂流速比例的加大,峰面积增大;在管长一定的情况下,冷却浴温度对峰面积没有显著影响。结论:反应浴温度、流动相与衍生试剂流速的比例、冷却浴温度等柱后衍生因素对伏格列波糖峰面积都有影响,因此在柱后衍生化分析时需要对这些因素进行优化,从而选择最优分析检测条件。
OBJECTIVE: To investigate the influence of post-column derivatization conditions on the HPLC determination of voglibose post-column derivatization, and provide the basis for the optimization of voglibose detection conditions. Methods: The effects of different reaction bath temperature, cooling bath temperature, derivatization reagent concentration and mobile phase flow rate / derivatization reagent flow rate on the post-column derivatization of voglibose were investigated. Results: Under the experimental conditions, the peak area of voglibose increased with the increase of the temperature of the reaction bath. In a certain range, the increase of the concentration of derivative reagent led to the increase of peak area of voglibose, Increasing the concentration of the derivatization reagent decreased the peak area. With the increase of mobile phase / derivatization ratio, the peak area increased. In the case of a certain tube length, the temperature of the cooling bath had no significant effect on the peak area. CONCLUSIONS: The post-column derivatization factors, such as the temperature of the reaction bath, the ratio of the mobile phase to the flow rate of the derivatizing reagent and the temperature of the cooling bath, affect the peak area of voglibose. Therefore, these factors need to be optimized in the post- Thus choose the optimal analysis of test conditions.