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作者基本按Mazier等(1983,1984),以间日疟原虫完整的肝内裂体增殖法培养恶性疟原虫红外期获得成功。该法用35mm的培养皿,每皿接种6×10~8的人体肝细胞作单层培养,在2次连续培养期间,用人工培养的配子体(来源于NF54和克隆7G8)喂饲的弗氏按蚊或斯氏按蚊唾液腺5～10对进行感染。用培养物与1例非洲成人高免疫血清和以1/2000伊文思蓝溶液作1:30稀释的荧光抗体结合物(Nordic)进行间接免疫荧光试验和/或吉氏液染色检查肝细胞内裂殖体。结果:感染达21小时,可见大量固定或不固定在细胞表面的子孢子,少数在细胞浆内形成3～4mμ的圆形荧光。感染后第3、5、6和7天在全部培养物中,细胞浆内可见到多数为圆形,少数为卵圆形,位置常与胞
According to Mazier et al. (1983, 1984), the author succeeded in culturing P. falciparum in the infrared phase with the complete intrahepatic septicemia multiplication method of Plasmodium vivax. The method used 35mm Petri dishes, each dish was inoculated with 6 × 10 ~ 8 human liver cells for monolayer culture, during two consecutive cultures, with artificial culture gametocytes (derived from NF54 and clone 7G8) fed Freunds Anopheles or Anopheles stephensi salivary glands 5 to 10 pairs of infection. Indirect immunofluorescence and / or Giemsa staining were used to examine the hepatocyte endo-fission by culture with one case of adult African high-immune serum and a 1:30 dilution of Nordic in 1/2000 Evans blue solution Colonies. Results: Infection up to 21 hours, a large number of fixed or not fixed in the cell surface sporozoites, a few formed in the cytoplasm of 3 ~ 4mμ circular fluorescence. On the 3rd, 5th, 6th and 7th days after infection, most of the cultures were found in the cytoplasm, most of which were round and few in oval,