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当归[Angelica sinensis(Oilv)Diels]为重要的中药材,常有“十方九当”之称,自古尤以甘肃秦州(今岷县、武都一带)所产为上品。我们于1980年4月开始选用甘肃岷县当归为材料,分别用根、叶、叶柄、子叶、下胚轴等器官诱导产生了愈伤组织和小植株(图1,2)。当归各器官产生愈伤组织的能力不同,其中由叶片产生的愈伤组织效果最差,根产生愈伤组织最快(8—10天)、频率最高(87％),继代培养中生长也最旺盛。
Angelica sinensis (Oilv) Diels is an important traditional Chinese medicinal material. It is often called “Shifangjiadang” and has been produced by Qinzhou (now Minxian, Wudu) in Gansu since ancient times. In April 1980, we started to select the angelica of Minxian County in Gansu Province as the material, and the callus and plantlets were induced by roots, leaves, petioles, cotyledons, hypocotyls and other organs respectively (Figures 1 and 2). Angelica sinensis has different ability to produce callus, of which the callus produced by the leaves is the worst, the callus produced the fastest (8-10 days), the highest frequency (87%), the growth of the subculture Most exuberant.