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目的探讨人参水提液(water extract of ginseng,WEG)对肿瘤相关巨噬细胞(tumor associated macrophages,TAMs)与肺癌A549细胞共培养体系肺癌A549细胞增殖、迁移及骨架蛋白F-actin表达等生物学行为影响。方法采用佛波酯(PMA)联合IL-4及IL-13诱导人急性白血病单核细胞株(THP-1)成为TAMs。以TAMs细胞上清与肺癌A549细胞共培养,模拟肿瘤微环境构建共培养模型。将细胞分为空白组(A549)、共培养组(TAMs+A549)、WEG高、中、低剂量组(TAMs+A549+WEG)。分别采用MTT法、实时细胞分析技术及高内涵细胞成像系统检测分析不同条件下A549细胞增殖、迁移和骨架蛋白F-actin表达情况。结果与空白组比较,共培养组A549细胞增殖明显增加,细胞迁移能力和细胞骨架面积均明显升高,差异有统计学意义(P<0.05);与共培养组比较,WEG高、中、低剂量组A549细胞增殖和迁移能力受抑制,细胞骨架面积和微丝条数减少,且呈剂量依赖性(P<0.05)。结论人参水提液在共培养体系中能有效抑制A549细胞增殖迁移能力,可能通过调节TAMs免疫活性而影响肿瘤细胞的生物学行为。
Objective To investigate the biological effects of water extract of ginseng (WEG) on the proliferation and migration of lung cancer A549 cells and the expression of F-actin in lung cancer A549 cells induced by tumor associated macrophages (TAMs) and lung cancer A549 cells Behavioral impact. Methods TMAs were induced by the combination of phorbol ester (PMA) and IL-4 and IL-13 in human acute leukemia monocytic cell line (THP-1). The TAMs cell supernatants were co-cultured with lung cancer A549 cells to simulate the tumor microenvironment and construct a co-culture model. The cells were divided into blank group (A549), co-culture group (TAMs + A549), WEG high, medium and low dose groups (TAMs + A549 + WEG). The proliferation, migration and expression of F-actin in A549 cells under different conditions were detected by MTT assay, real-time cell analysis and high-content cell imaging system respectively. Results Compared with the blank group, the proliferation of A549 cells in co-culture group was significantly increased, and the migration ability and cytoskeleton area were significantly increased (P <0.05). Compared with the co-culture group, the WEG high, middle and low doses The proliferation and migration of A549 cells were inhibited, the cytoskeleton area and the number of micro-filaments were decreased, and in a dose-dependent manner (P <0.05). Conclusion Ginseng aqueous extract can effectively inhibit the proliferation and migration of A549 cells in co-culture system and may affect the biological behavior of tumor cells by regulating the immunological activity of TAMs.