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目的:观察木通所致大鼠急性肾损伤的远期效应(功能及组织形态学改变特点).方法:用不同剂量木通水煎剂(50 g·kg~(-1)·d~(-1)×7 d、30 g·kg~(-1)·d~(-1)×7d、20 g·kg~(-1)·d~(-1)×15 d)给大鼠灌胃,建立大鼠急性肾损伤动物模型后,检测实验开始(给药结束之日)、1月、3月、6月时各种肾功能指标,及肾组织形态学的变化.结果:1)木通所致大鼠急性肾损伤的近期功能改变为,氮质血症、低渗尿、低分子蛋白尿、糖尿、尿NAG酶升高.组织形态学改变主要为急性肾小管坏死,以皮髓交界区小管损伤为主.2)实验1月、3月木通组大鼠的肾功能逐渐恢复,皮髓交界区小管病变逐渐恢复,小管上皮细胞胞浆内可见嗜碱性物质沉积,间质浸润细胞较少,未见间质慢性炎症和纤维化等慢性化病变.3)实验6月大鼠发生肾脏间质肿瘤样增生及全身性肿瘤.结论:1)大剂量木通可导致大鼠急性肾功能衰竭.2)木通所致大鼠急性肾损伤远期其肾功能及小管损伤可逐渐恢复,未见明显小管间质慢性化病变.3)木通具有致肿瘤作用.
OBJECTIVE: To observe the long-term effects (function and histomorphological changes) of rats with acute kidney injury induced by wood exposure. Methods: Different dosages of wood decoction (50 g·kg~(-1)·d~( -1)×7 d,30 g·kg -1 ,d -1 ,7 d,20 g·kg -1 ,d -1 ×15 d for rats Stomach, after establishment of an animal model of acute kidney injury in rats, various renal function indexes and changes in renal tissue morphology at the beginning of the experiment (on the end of the administration), January, March, and June of the test were examined. Results: 1) The recent functional changes of acute kidney injury induced by chlorhexidine chloride in rats are: azotemia, hypoosmotic, low-molecular-weight proteinuria, glucosuria, and elevated urinary NAG enzyme. Histomorphological changes are mainly acute tubular necrosis. The tubular lesions in the borderline of the medullary region are predominant. 2) The kidney function of rats in the Moutong group gradually recovers in the first and third months of the experiment. The tubular lesions in the junction of the pulp and the marrow gradually recover, and the basophilic material deposits are observed in the cytoplasm of the tubule epithelium. The infiltrating cells were few and no chronic diseases such as interstitial chronic inflammation and fibrosis were observed. 3) Rats with renal interstitial neoplasms and systemic neoplasms developed in June. Conclusions: 1) Large doses of woodblock can lead to large Rat Acute Renal Failure. 2) Mutong Acute kidney injury induced by long-term renal function and tubular damage gradually, without any significant chronic tubulointerstitial lesions .3) Akebia having oncogenic effect.