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抽滤是实验中除菌的方法之一,在细胞培养中广泛使用。抽滤所用的装置一般有两种:一是用真空泵抽滤,此法在抽滤过程中油蒸汽易回流而污染滤器和滤液。另一种是用三通水泵抽滤,用一三通管将自来水管与抽滤瓶、排水管连接,利用水流的空吸作用对溶液进行抽滤灭菌,但水很容易通过三通管道回流至抽滤瓶中,污染滤液而前功尽弃。为了解决这一问题,我们经过试验,在抽滤瓶与三通之间串接一个缓冲瓶,这样在抽滤时就避免了因水
The suction filtration is one of the methods of sterilizing in the experiment, which is widely used in cell culture. There are generally two types of devices for suction filtration: one is suction filtration with a vacuum pump, the method is easy to return the oil vapor and pollute the filter and the filtrate during the suction filtration process. The other is the use of three-way pump suction filtration, with a three-way pipe to the suction filter bottles, drains connected to the use of water suction suction sterilization of the solution, but the water is easily through the three-way pipe Reflux to the suction flask, contaminated filtrate and tried out. In order to solve this problem, we tested to conclude a buffer bottle between the suction filter and the tee, so as to avoid the water