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目的:调查深圳入境旅客西尼罗病毒的感染情况,探讨该病毒合适的检测技术,初步对入境旅客传入该病的风险进行评估。方法:采用基于入境旅客的血清流行病学调查方法(现况调查),以ELISA检测法检测目标人群血清特异性IgM及IgG抗体,检出阳性则进一步以荧光RT-PCR检测病毒核酸以确诊,以此评估入境旅客的感染情况。结果:共收集到口岸截获发热旅客的血清154份,入境体检旅客血清293份。发热旅客WNV-IgG阳性率为31.8%,体检旅客WNV-IgG阳性率为36.5%.对抗体检测阳性血清进行荧光RT-PCR检测,未发现阳性病例。结论:所调查的入境旅客中,西尼罗病毒特异性IgG总体阳性率为38.9%,群体免疫水平较高,西尼罗热通过旅客传入我国的风险较低。
Objective: To investigate the infection status of West Nile virus inbound travelers in Shenzhen and to explore the suitable detection technology of this virus to assess the risk of imported travelers entering the disease initially. Methods: Serum-specific IgM and IgG antibodies were detected by enzyme-linked immunosorbent assay (ELISA) in serum samples of inbound travelers. The positive samples were detected by RT-PCR and confirmed by real-time PCR. In order to assess the incidence of inbound travelers. Results: A total of 154 cross-sectional seizures of febrile travelers and 293 sera of inbound medical visitors were collected. The positive rate of WNV-IgG in fever travelers was 31.8%, and the positive rate of WNV-IgG in health check-ups was 36.5% .Fluorescent RT-PCR was used to detect the positive serums of antibody test. No positive cases were found. CONCLUSIONS: The overall positive rate of West Nile virus-specific IgG was 38.9% among the infested travelers surveyed, with a high level of population immunity and a lower risk of West Nile fever reaching our country through travelers.