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目的:为了了解庚型肝炎病毒(HGV)在我国的感染状况,得到一较好的HGV检测方法.方法:采用两对GBV-C(HGV)NS_3区引物,用逆转录-套式-聚合酶链反应(RT-nested-PCR)和ELISA方法对67例非甲乙丙丁戊型急性肝炎患者血清进行了检测.结果:发现17例呈NGV RNA阳性,阳性率为25.4%,PCR与ELISA法进行抗-HGVIgG的检测的阳性符合率为18%,同时对HGV的检测方法进行讨论.结论:西安市急性肝炎患者中存在着一定程度的HGV感染.PCR特别是逆转录套式PCR方法在目前来说无疑是进行HGV检测特异性和灵敏度均较好的选择,可广泛用于临床和流行病学研究.
Objective: In order to understand the status of hepatitis G virus (HGV) infection in China, a better HGV detection method was obtained.Methods: Two pairs of GBV-C (HGV) Serum samples from 67 cases of non-B hepatitis A virus were detected by RT-nested-PCR and ELISA.Results: 17 cases were positive for NGV RNA and the positive rate was 25.4% -HGVIgG positive detection rate was 18%, and HGV detection methods were discussed.Conclusion: There is a certain degree of HGV infection in patients with acute hepatitis in Xi’an.PCR, especially reverse transcriptase nested PCR method for the moment Undoubtedly the specificity and sensitivity of HGV detection are better choices, can be widely used in clinical and epidemiological studies.