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目的:测定并比较不同产地和不同采收期辽东楤木叶中3-O-β-D-葡萄吡喃糖基-(1→3)-β-D-葡萄吡喃糖基-(1→3)-β-D-葡萄吡喃糖基-齐墩果酸-28-O-β-D-吡喃葡萄糖苷(楤木皂苷TTP)和3-O-[β-D-葡萄吡喃糖基(1→2)]-[β-D-葡萄吡喃糖基(1→3)]-β-D-葡萄吡喃糖基-齐墩果酸-28-O-β-D-葡萄吡喃糖酯(楤木皂苷V)的含量。方法:采用高效液相色谱蒸发光散射检测(HPLC-ELSD)法,使用Diamonsil C_(18)(2)色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-水(31∶69),流速1.0 m L·min~(-1),柱温30℃,蒸发光散射检测器漂移管温度110℃,载气(N2),流速3.2 m L·min~(-1)。结果:楤木皂苷TTP和楤木皂苷V分别在进样量0.51~2.55μg和0.50~4.00μg线性关系良好,相关系数分别为0.999 8和0.999 2。结果表明,不同产地和不同采收期辽东楤木叶中楤木皂苷TTP和楤木皂苷V的含量有所差异,可能与辽东楤木分布区域、生长季节及叶片生理状态有关,由实验结果可以看出,两种皂苷类成分大多在7,8月的含量相对较高,部分地区9月含量较高。结论:该方法简单、快捷、准确,可以同时测定辽东楤木叶中楤木皂苷TTP和楤木皂苷V的含量,为辽东楤木叶质量控制提供实验依据。
OBJECTIVE: To determine and compare the inhibitory effects of 3-O-β-D-glucopyranosyl- (1 → 3) -β-D-glucopyranosyl- (1 → 3) -β-D-glucopyranosyl-oleanolic acid-28-O-β-D-glucopyranoside (saponin TTP) and 3-O- [β-D-glucopyranose (1 → 2)] - [β-D-glucopyranosyl (1 → 3)] - β-D-glucopyranosyl-oleanolic acid -28-O-β-D-glucopyranosyl Amylalcohol ester (albino saponin V) content. Methods: The HPLC-ELSD method was used to determine the content of nitroblue tetrazolium (DEAE) in blood by HPLC-ELSD. Diamonsil C 18 column (4.6 mm × 250 mm, 5 μm) The flow rate was 1.0 m L · min -1, the column temperature was 30 ℃, the evaporating light scattering detector drift tube temperature was 110 ℃, the carrier gas was N2 and the flow rate was 3.2 m L · min -1. Results: The linear correlations between alfalfa saponin TTP and alder saponin V were 0.51 ~ 2.55μg and 0.50 ~ 4.00μg, respectively, with correlation coefficients of 0.999 8 and 0.999 2, respectively. The results showed that the contents of alfalfa saponins TTP and albino saponin V in Alnus mandshurica of different producing areas and different harvesting periods were different, which may be related to the distribution area, growth season and physiological status of Alnus mandshurica, See, most of the two saponins in July and August the content is relatively high, in some areas in September higher levels. Conclusion: The method is simple, rapid and accurate. The content of alfalfa saponins TTP and albino saponin V in alder leaves of Alnus mandshurica could be determined at the same time, providing the experimental evidence for the quality control of alder leaves of Alnus mandshurica.