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目的:探讨声门上型喉鳞状细胞癌组织中乳腺癌转移抑制基因(BRMS1)的蛋白表达以及BRMS1基因启动子区域甲基化情况及其临床意义。方法:采用Western blotting法检测70例声门上型喉癌组织原发灶、60例癌旁正常喉黏膜组织和44例颈部淋巴结转移灶中BRMS1蛋白的表达情况;甲基化特异聚合酶链反应(MSP)法检测上述组织中的BRMS1基因启动子区域甲基化情况,探讨BRMS1基因启动子甲基化与其基因表达的相关性;分析BRMS1蛋白表达及BRMS1基因启动子甲基化情况与患者的临床分期、病理分级、颈部淋巴结转移等方面的相关分析。结果:Western blotting检测发现声门上型喉癌原发灶、癌旁正常喉黏膜组织和颈部淋巴结转移灶均有BRMS1蛋白表达,在癌组织及转移淋巴结中BRMS1蛋白表达下调(P<0.05)。MSP法检测发现BRMS1基因启动子区甲基化,在BRMS1蛋白低表达患者的原发灶中检测到34例BRMS1基因启动子区甲基化,44例BRMS1蛋白低表达的颈部淋巴结转移灶中检测到32例BRMS1基因启动子区甲基化,60例癌旁正常喉黏膜组织中均未检测到BRMS1基因启动子区甲基化,统计学分析结果表明在声门上型喉癌中BRMS1基因启动子甲基化与其基因表达下调密切相关(rs=0.66,P<0.05)。结论:声门上型喉癌组织中BRMS1蛋白表达下调。BRMS1蛋白表达下调与声门上型喉癌的P-TNM分期、病理分化程度和颈部淋巴结转移密切相关。BRMS1基因启动子甲基化与声门上型喉癌组织中BRMS1基因表达下调相关,也可能是声门上型喉癌组织中BRMS1基因表达下调的原因之一。
Objective: To investigate the protein expression of breast cancer metastasis suppressor gene (BRMS1) and the promoter methylation of BRMS1 gene in supraglottic laryngeal squamous cell carcinoma and its clinical significance. Methods: Western blotting was used to detect the expression of BRMS1 protein in 70 cases of supraglottic laryngeal carcinoma, 60 cases of normal laryngeal mucosa and 44 cases of cervical lymph node metastasis. Methylation-specific polymerase chain (MSP) was used to detect the methylation status of BRMS1 promoter in the above tissues, and to explore the relationship between BRMS1 promoter methylation and its gene expression. The BRMS1 protein expression and promoter methylation of BRMS1 gene were compared with those in patients The clinical stage, pathological grade, cervical lymph node metastasis and other aspects of the correlation analysis. Results: The results of Western blotting showed that the expression of BRMS1 protein was found in the supraglottic laryngeal carcinoma, adjacent normal laryngeal mucosa and cervical lymph node metastasis. The expression of BRMS1 was down-regulated in both cancerous tissues and metastatic lymph nodes (P <0.05) . Methylation of the promoter region of BRMS1 gene was detected by MSP assay, methylation of BRMS1 gene promoter region was detected in 34 cases of primary BRMS1 low expression, and 44 cases of cervical lymph node metastasis with low expression of BRMS1 protein Detection of BRMS1 promoter methylation in 32 cases and no methylation of BRMS1 promoter in 60 cases of paracancerous normal laryngeal mucosa tissues. Statistical analysis showed that in the supraglottic laryngeal carcinoma, the BRMS1 gene Promoter methylation was closely related to its gene expression (rs = 0.66, P <0.05). Conclusion: The expression of BRMS1 protein in supraglottic laryngeal carcinoma was down-regulated. Down-regulation of BRMS1 expression is closely related to P-TNM stage, pathological differentiation and lymph node metastasis in supraglottic laryngeal carcinoma. The promoter methylation of BRMS1 gene is associated with the down-regulation of BRMS1 gene expression in supraglottic laryngeal carcinoma and may be one of the reasons for the down-regulation of BRMS1 gene expression in supraglottic laryngeal carcinoma.