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为了探索非生物胁迫下硅对辣椒Ca MADS-box基因表达的影响,以豫椒101为材料,在对辣椒Ca MADS-box基因片段同源克隆的基础上,对其编码的部分蛋白进行氨基酸同源性分析、理化性质预测、亚细胞定位预测和进化树分析,探讨了硅处理后辣椒Ca MADS-box基因在高温胁迫、盐胁迫下表达量的变化,结果表明,克隆得到的Ca MADSbox基因所编码的蛋白为亲水性蛋白,含有MADS结构域,属于MADS基因家族,亚细胞定位在细胞核中,分子进化树表明其与茄属和烟草属亲缘关系最近,相似性达到67%。荧光定量分析表明,Ca MADS-box基因在高温胁迫和盐胁迫后表达量都呈现出先升高后下降的模式,高温胁迫下48 h达到峰值,而盐胁迫在24 h达到峰值,硅处理能够诱导Ca MADS-box基因表达,在高温胁迫和盐胁迫下都在12 h达到高峰值,这表明Ca MADS-box是一个硅快速响应基因,推测硅处理在缓解辣椒高温胁迫和盐胁迫等非生物胁迫方面具有重要作用。
In order to explore the effect of silicon on gene expression of Ca MADS-box in pepper under abiotic stress, we constructed the pepper Ca MADS-box gene fragment based on the homologous cloning of Ca MADS- Source analysis, physical and chemical properties prediction, subcellular localization prediction and evolutionary tree analysis. The changes of Ca MADS-box gene expression under high temperature stress and salt stress after silicon treatment were investigated. The results showed that the cloned Ca MADSbox gene The encoded protein is a hydrophilic protein containing the MADS domain, belonging to the MADS gene family. The subcellular localization of the subcellular localization in the nucleus showed that the molecular phylogenetic tree showed the closest relationship with Solanum and Nicotiana, with a similarity of 67%. Fluorescence quantitative analysis showed that Ca MADS-box gene expression increased firstly and then decreased after high temperature stress and salt stress, reached the peak at 48 h under high temperature stress, but reached the peak at 24 h under salt stress, and the silicon treatment could induce Ca MADS-box gene expression peaked at 12 h under both high temperature stress and salt stress, indicating that Ca MADS-box is a silicon rapid response gene. It is speculated that silicon treatment can alleviate abiotic stresses such as high temperature stress and salt stress Has an important role.