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目的探讨人乳头瘤病毒１６（ＨＰＶ１６）与胃癌发生的关系。方法用聚合酶链式反应（ＰＣＲ）扩增出ＨＰＶ１６早期区Ｅ６的１２０ｂｐＤＮＡ片段，扩增产物与５＇末端３２Ｐ标记的特异性寡核苷酸探针进行斑点杂交及放射性自显影，用此方法对３４６份新鲜的及福尔马林固定石蜡包埋的胃组织标本中ＨＰＶ１６ＤＮＡ进行检测。结果胃腺癌、癌旁粘膜、胃局部淋巴结及正常胃粘膜组织中ＨＰＶ１６的检出率分别为：２２．７２％（３０／１３２）、５．８８％（４／６８）、０％（０／４２）、２．８８％（３／１０４），胃癌组ＨＰＶ１６的检出率高于其他各组，统计学上差异均有显著性（Ｐ＜０．０１）。结论ＨＰＶ１６可感染胃粘膜上皮细胞，可能是胃癌的致癌因素之一。
Objective To investigate the relationship between human papillomavirus 16 (HPV16) and gastric cancer. Methods The 120 bp DNA fragment of E6 in the early stage of HPV16 was amplified by polymerase chain reaction (PCR). Dot blotting and autoradiography were performed on the amplification product and specific oligonucleotide probe labeled with 32P at the 5 ’end. HPV16DNA was detected in 346 fresh and formalin-fixed paraffin-embedded gastric tissue specimens. Results The positive rates of HPV16 in gastric adenocarcinoma, paraneoplastic mucosa, gastric local lymph node and normal gastric mucosa were 22.72% (30/132), 5.88% (4/68), 0% (0 / 42) and 2.88% (3/104) respectively. The detection rate of HPV16 in gastric cancer group was higher than that in other groups, with statistical significance (P <0.01). Conclusion HPV16 can infect gastric mucosal epithelial cells and may be one of the carcinogenic factors of gastric cancer.