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采用免疫组化ＡＢＣ法测定了用水浸、温度、酸、碱、腐败、霉变等理化因素处理后的人体组织的ＡＢＨ物质含量变化。结果显示：水浸２１天的皮肤、１００℃处理４０分钟的舌、０．２ｍｏｌＬＨＣｌ和０．２ｍｏｏｌ／ＬＮａＯＨ浸泡４８小时的皮肤与舌以及１２ｍｏｌ／ＬＨＣｌ和１２ｍｏｌ／ＬＮａＯＨ浸泡３０分钟的皮肤．它们的ＡＢＨ物质有不同程度的减少，仍能正确判定其ＡＨＯ型。腐败与霉变２１天的胰腺组织，血管内皮尚含ＡＢＨ物质，依然能正确判定其ＡＢＯ型，不受霉菌和细菌Ｂ、Ｈ物质的影响。
The changes of ABH content in human tissues treated with physicochemical factors such as water immersion, temperature, acid, alkali, decay, mildew and so on were determined by immunohistochemical ABC method. The results showed that the skin immersed for 21 days, the tongue treated for 40 minutes at 100 ° C, the skin and tongue immersed for 48 hours with 0.2 mol of HCl and 0.2 mol / L NaOH, and the skin immersed for 30 minutes with 12 mol / LHCl and 12 mol / LNaOH. Their ABH substances with varying degrees of reduction, still can correctly determine the AHO type. Corruption and mildew 21 days of pancreatic tissue, vascular endothelial still contains ABH substances, still can correctly determine the ABO type, not mold and bacteria B, H substances.