论文部分内容阅读
目的:建立快速测定陈皮中橙皮苷、川陈皮素及橘红素含量的方法。方法:采用Waters Acquity UPLC系统;色谱柱为Waters BEH C18柱(2.1mm×50mm,1.7μm);流动相为乙腈-水,梯度洗脱;体积流量0.5ml.min-1;柱温:30℃;检测波长为326nm。结果:线性范围内橙皮苷、川陈皮素及橘红素的峰面积与相应质量浓度呈良好线性关系,加样回收率分别为98.23%~101.64%、98.20%~102.06%、98.45%~101.53%。结论:本方法快速、简便、准确,可用于陈皮药材的质量控制。
OBJECTIVE: To establish a rapid method for the determination of hesperidin, nobiletin and nectarine in dried tangerine peel. Methods: The Waters Acquity UPLC system was used. The column was a Waters BEH C18 column (2.1 mm × 50 mm, 1.7 μm). The mobile phase was acetonitrile-water with gradient elution. The volume flow rate was 0.5 ml · min -1. ; Detection wavelength of 326nm. Results: The linear range of the hesperidin, nobiletin and the concentration of mandarin peak area and the corresponding mass concentration showed a good linear relationship, the recoveries were 98.23% ~ 101.64%, 98.20% ~ 102.06%, 98.45% ~ 101.53% . Conclusion: The method is rapid, simple and accurate and can be used for quality control of dried tangerine peel.