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以含有200 mmol·L-1 NaCl的1/2 Hoagland营养液处理‘寒富’二倍体苹果及其同源四倍体幼苗8 d,分别在处理0、2、4、6和8 d时进行叶片相关生理指标及水通道蛋白相关基因表达水平的测定,比较分析两者的耐盐性差异。结果表明:盐胁迫下四倍体的形态表现优于二倍体;四倍体和二倍体的叶片相对含水量一直在下降,二倍体下降幅度大于四倍体,丙二醛含量和脯氨酸的积累随胁迫时间的延长而增加,二倍体含量始终高于四倍体;NaCl处理8 d时,二倍体的叶片相对含水量比四倍体低8%,丙二醛含量比四倍体多4.466 nmol·g-1FW,脯氨酸含量为四倍体的1.18倍;盐胁迫下两者叶片中水通道相关蛋白基因Md PIP1;1、Md PIP2;1、Md TIP1;1和Md TIP2;1的表达量均出现先下降后上升趋势,且四倍体均高于二倍体,尤其在处理24 h时差异最显著。四倍体比二倍体具有更强的耐盐性,可能与盐胁迫下四倍体相关水通道蛋白基因表达水平较高有关。
The cold-rich diploid apple and its autotetraploid seedlings were treated with ½ Hoagland nutrient solution containing 200 mmol·L -1 NaCl for 8 d, and were treated for 0, 2, 4, 6 and 8 d Leaf physiological index and aquaporin related gene expression levels were measured, and the differences of salt tolerance between the two were compared. The results showed that the morphology of tetraploids under salt stress was better than that of diploids. The relative water content of tetraploids and diploids decreased continuously, and the diploid decreased more than that of tetraploids. The contents of malondialdehyde The accumulation of amino acid increased with prolonging of stress time, and the content of diploid was always higher than that of tetraploid. The relative water content of diploid was 8% lower than that of tetraploid at 8 d of NaCl treatment. Tetraploid 4.466 nmol · g-1FW, proline content of tetraploid 1.18 times; Under salt stress leaves of the water channel-related protein gene Md PIP1; 1, Md PIP2; 1, Md TIP1; The expression level of Md TIP2; 1 firstly descended and then increased, and the tetraploids were higher than diploid, especially the difference was most significant at 24 h. Tetraploid is more salt tolerant than diploid and may be related to the higher tetraploid aquaporin gene expression under salt stress.