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目的:研究中药制剂中枸橼酸西地那非的预处理方法。方法:用水对样品进行提取,提取液用氨水碱化(pH=9),用氯仿萃取,水浴蒸干,残渣用甲醇溶解;采用反相高效液相色谱法进行测定,色谱条件:色谱柱为ODSC18柱(250 mm×4.6 mm,5μm),流动相为甲醇-水-冰醋酸-三乙胺(60∶40∶0.8∶0.3,v/v),流速为0.8 ml/min,检测波长为290 nm,进样量为10μl。结果:平均加样回收率为100.6%,RSD为1.45%(n=6)。结论:本研究结果准确,方法可行。
Objective: To study the pretreatment method of sildenafil citrate in traditional Chinese medicine preparations. Methods: Samples were extracted with water. The extracts were basified with ammonia (pH = 9). The extracts were extracted with chloroform. The bath was evaporated to dryness and the residue was dissolved with methanol. The determination was carried out by RP-HPLC. The chromatographic conditions were: ODSC18 column (250 mm×4.6 mm, 5 μm), mobile phase methanol-water-glacial acetic acid-triethylamine (60:40:0.8:0.3, v/v), flow rate 0.8 ml/min, detection wavelength 290 Nm, injection volume 10 μl. Results: The average recovery was 100.6% with an RSD of 1.45% (n=6). Conclusion: The results of this study are accurate and feasible.