目的 :研究 5α-还原酶 ( 型 )的基因表达方法及其 m RNA在附睾各段内的分布。方法 :采用异硫氰酸胍法提取总 RNA,“随机引物”法制备 α- 32 P标记的 5α-还原酶 c DNA探针 ,Northernblot法检测附睾各部分基因表达的变化。结果 :附睾各部分均有表达 ,其中头部表达最高 ,体、尾部依次降低。结论 :提示附睾头部 5α-还原酶 m RNA表达量高与其功能有关 Objective: To study the gene expression pattern of 5α-reductase (type) and the distribution of m RNA in the segments of epididymis. Methods: The total RNA was extracted by guanidine isothiocyanate method. The α-32 P-labeled 5α-reductase c DNA probe was prepared by “random primer” method. The expression of some genes in epididymis was detected by Northern blot. Results: All parts of epididymis were expressed, of which the highest expression of the head, followed by body and tail decreased. Conclusion: The high expression of 5α-reductase mRNA in the head of the epididymis is related to its function