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AIM:To determine the role of CD133 in cholangiocarcinoma progression. METHODS:CD133 protein expression was evaluated by immunohistochemistry in 34 cholangiocarcinoma specimens.In addition,proliferation,chemoresistance and invasive properties of CD133-enriched(CD133 + ) and CD133-depleted(CD133 )RMCCA1 cholangiocarcinoma cells were studied and compared. RESULTS:Strong CD133 expression was observed in 67.6%(23/34)of the cholangiocarcinoma specimens. Strong expression of CD133 was significantly associated with nodal metastasis(P=0.009)and positive surgical margin status(P=0.011).In the in vitro study, both the CD133 + and CD133 cells had similar proliferation abilities and resistance to chemotherapeutic drugs.However,the CD133 + cells had a higher invasive ability compared with CD133 cells. CONCLUSION:CD133+cells play an important role in the invasiveness of cholangiocarcinoma.Targeting of the CD133+cells may be a useful approach to improve treatment against cholangiocarcinoma.
METHODS: CD133 protein expression was evaluated by immunohistochemistry in 34 cholangiocarcinoma specimens. In addition, proliferation, chemoresistance and invasive properties of CD133-enriched (CD133 +) and CD133-depleted (CD133) Strong CD133 was significantly associated with nodal metastasis (P = 0.009) and positive surgical margin status (P = 0.011) .In the in vitro study, both the CD133 + and CD133 cells were similar proliferation abilities and resistance to chemotherapeutic drugs. However, the CD133 + cells had a higher invasive ability than with CD133 cells. CONCLUSION: CD133 + cells play an important role in the invasiveness of cholangiocarcinoma. Targeting of the CD133 + cells may be a useful approach to improve treatment against cholangiocarcinoma.