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目的 观察急性冠脉综合征 (ACS)患者单核细胞对IL -18的表达及氟伐他汀对其抑制作用。方法 分离ACS患者外周血单核细胞 ,不同浓度氟伐他汀干预培养 ,逆转录聚合酶链反应 (RT -PCR)方法检测单核细胞对IL -18mRNA的表达。结果 ACS患者外周血单核细胞可表达IL -18mRNA ,0 1μmol·L-1 、1 0 μmol·L-1 浓度氟伐他汀干预时 ,IL -18mRNA光密度分别从 5 4 1±9 9降至 5 0 1± 12 6(P <0 0 5 )、41 2± 9 8(P <0 0 1) ,呈剂量依赖性。结论 ACS患者外周血单核细胞可表达IL -18mRNA ,且氟伐他汀对其呈剂量依赖性抑制
Objective To investigate the expression of interleukin-18 (IL-18) by monocytes in patients with acute coronary syndrome (ACS) and the inhibitory effect of fluvastatin. Methods Peripheral blood mononuclear cells (PBMCs) were isolated from patients with ACS. Fluvastatin with different concentrations was intervened and cultured. The expression of IL-18 mRNA in mononuclear cells was detected by reverse transcriptase polymerase chain reaction (RT-PCR). Results IL-18 mRNA could be expressed in peripheral blood mononuclear cells of patients with ACS. The optical density of IL-18 mRNA decreased from 541 ± 9 9 in the presence of 0 1 μmol·L-1 and 10 μmol·L-1 fluvastatin, respectively 5 0 1 ± 12 6 (P 0 05) and 41 2 ± 9 8 (P 0 01) in a dose-dependent manner. Conclusion Peripheral blood mononuclear cells of patients with ACS can express IL-18 mRNA, and fluvastatin can inhibit it in a dose-dependent manner