土壤碳矿化速率及温度敏感性是研究陆地生态系统碳循环的重要指标,以往研究多集中在表层土壤,但不同深度土壤属性及碳质量具有显著的差异。以亚热带马尾松/木荷人工混交林红壤为研究对象,选择0~10 cm、10~30 cm、30~60 cm和60~100 cm四种深度土壤,设置葡萄糖添加(G+)和空白对照(CK)两组处理,进行周期性变温(4、14、22和30℃)培养,并利用自主研发设备测定第1、3、7、14、21和28天5~30℃模拟昼夜周期性变温条件下的土壤碳矿化速率,研究添加葡萄糖对不同红壤碳矿化速率及其温度敏感性(Q10)的影响。结果表明:无葡萄糖添加时,随着土壤深度和培养时间增加,土壤碳矿化速率显著降低,但Q10无显著差别,培养后期底物供给不足限制了土壤碳矿化速率。葡萄糖添加后,随着土壤深度增加,土壤碳矿化速率及Q10均显著增加,浅层土壤响应较快,深层土壤响应慢但增幅更大。培养末期深层土壤碳矿化速率甚至高于表层土壤。不同深度土壤微生物含量及其群落结构组成是土壤碳矿化速率及其Q10响应差异的主要影响因素。 Soil carbon mineralization rate and temperature sensitivity are important indices for studying the carbon cycle of terrestrial ecosystems. In the past, most studies focused on surface soils, but there were significant differences in soil properties and carbon quality at different depths. Taking Pinus massoniana / Schima superba mixed forest red soil as the research object, the soil depths of 0 ~ 10 cm, 10 ~ 30 cm, 30 ~ 60 cm and 60 ~ 100 cm were selected. CK). The cells were cultured periodically (4, 14, 22 and 30 ℃), and the temperature was set at 5 ~ 30 ℃ on the 1st, 3rd, 7th, 14th, 21st, On the soil carbon mineralization rate, the effects of glucose on the carbon mineralization rate and its temperature sensitivity (Q10) of different red soils were studied. The results showed that with the addition of glucose, soil carbon mineralization rate decreased significantly with the increase of soil depth and culture time, but no significant difference was found in Q10. The insufficient supply of substrate at the end of cultivation limited the soil carbon mineralization rate. With the increase of soil depth, the soil carbon mineralization rate and Q10 increased significantly after the addition of glucose. The response of shallow soil was quick, but that of deep soil was slower but increased more. At the end of cultivation, the soil carbon mineralization rate was even higher than that of the surface soil. Soil microbial biomass and its community composition at different depths were the main influencing factors of soil carbon mineralization rate and Q10 response.